A Galegae. Orientalis L. gibberellin receptor, GoGID, is involved in GA signal and over-expression enhanced GA-signaling in tobacco
Jun Li, Xuemin Wang*, Hongwen Gao, Jishan Jiang
Department of Forage Germplasm Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
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Gibberellin(GA) is a well-known phytohormone that involves a wide range of plant growth, development, and environmental responses, including seed germination, stem elongation, leaf expansion, and flower and seed development. Gibberellin receptor perceive GA and transmit to cause GA-regulated reactions. In this study, an ortholog of GA receptor gene, termed GoGID, was isolated from G. orientalis. The 1732-bp full-length GoGID had a 1035-bp ORF, which encodes a peptide of 344 amino acids. The deduced amino acid sequence of GoGID protein shared high identity with other GIDs, and have conserved amino acid that essential for maintaining the GA-binding activity. The transcript of GoGID was abundant in leaves compared with roots and stems. Expression of GoGID was shown to be largely upregulated by GA3 and IAA. Overexpression of GoGID in transgenic tobacco plants resulted in rapid growth, increased leaf area, and stem elongation. These result demonstrated that GoGID function as GA receptor enhance GA-signaling and have potential role in genetic engineering for forage improvement.
Key Words: Galegae. Orientalis L, gibberellin receptor, gene expression, Transgenic tobacco